FENS Forum 2006

FENS Forum 2006 - Vienna

For lectures, symposia and workshops, time indicates the beginning of the session.
For posters, authors are expected to be in attendance at their posters at the time indicated.

First author: Banks, Duncan (poster)

Poster board 442 - Mon 10/07, 16:00 - Hall Y
Session 130 - Alzheimer's disease II
Abstract A130.2, published in FENS Forum Abstracts, vol. 3, 2006.
Ref.: FENS Abstr., vol.3, A130.2, 2006

Author(s) Banks D. & Stewart M. G.

Addresse(s) Biol. Sci., Open Univ., Milton Keynes, UK

Title Colostrinin™ (CLN) alleviates beta amyloid-induced (AB) memory impairment in day-old chicks (Gallus domesticus).

Text Colostrinin is a biologically active proline-rich polypeptide complex being developed by ReGen Therapeutics Plc (London, UK) that has therapeutic potential for a number of human diseases particularly Alzheimer’s. When injected into the mesopallium intermediomediale (IMM), an area that plays a key role in the initial stages of memory formation in the chick, CLN has a strong effect on memory enhancement when compared with control i. c. saline (SAL) injections (Stewart MG, & Banks D, 2006, Neurobiol. Learn. Memory, in press). The present experiments were designed to examine the efficacy of CLN to alleviate beta amyloid-(AB) -induced memory impairment using a single one-trial learning paradigm, avoidance of a bitter tasting substance (methylanthranilate, MeA). Day-old chicks were placed in pairs in aluminium pens kept at 25-28oC, illuminated with a 25W red bulb and after 1h of equilibration were pre-trained by two 10s presentations of a dry white bead. Only chicks that pecked the bead twice were included in subsequent experiments. Chicks were then trained with a chrome bead dipped in 100% MeA and those that pecked the bead displayed a disgust reaction with long-term memory indicated by their subsequent avoidance of pecking at a similar but dry bead 24h later. Colostrinin (100 microg in 0.1 ml 0.9% sterile SAL) was injected i. p. and bilateral intracranial injections of ‘aged'AB 12-28 (2.5 microl/hemisphere) were made into the IMM. 24 hours after training using 100% MeA, chicks were tested for memory retention by presentation of a dry chrome bead and birds scored as avoid (indicating memory retention) or peck (amnestic). The percentage avoidance of chicks untreated with AB or CLN was 82% and those injected i. c. with AB and i. p. SAL, memory retention fell to 52%. However, in birds injected with i. p. CLN and i. c. AB the percentage avoidance for the bead rose to 84% (Chi=55.63; p = 0.001) demonstrating that colostrinin can alleviate AB-induced memory impairment. Funding from ReGen.

Theme Neurological and psychiatric conditions
Neurodegenerative disorders / Alzheimers disease: Other

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Author(s)	Banks D. & Stewart M. G.
Addresse(s)	Biol. Sci., Open Univ., Milton Keynes, UK
Title	Colostrinin™ (CLN) alleviates beta amyloid-induced (AB) memory impairment in day-old chicks (Gallus domesticus).
Text	Colostrinin is a biologically active proline-rich polypeptide complex being developed by ReGen Therapeutics Plc (London, UK) that has therapeutic potential for a number of human diseases particularly Alzheimer’s. When injected into the mesopallium intermediomediale (IMM), an area that plays a key role in the initial stages of memory formation in the chick, CLN has a strong effect on memory enhancement when compared with control i. c. saline (SAL) injections (Stewart MG, & Banks D, 2006, Neurobiol. Learn. Memory, in press). The present experiments were designed to examine the efficacy of CLN to alleviate beta amyloid-(AB) -induced memory impairment using a single one-trial learning paradigm, avoidance of a bitter tasting substance (methylanthranilate, MeA). Day-old chicks were placed in pairs in aluminium pens kept at 25-28oC, illuminated with a 25W red bulb and after 1h of equilibration were pre-trained by two 10s presentations of a dry white bead. Only chicks that pecked the bead twice were included in subsequent experiments. Chicks were then trained with a chrome bead dipped in 100% MeA and those that pecked the bead displayed a disgust reaction with long-term memory indicated by their subsequent avoidance of pecking at a similar but dry bead 24h later. Colostrinin (100 microg in 0.1 ml 0.9% sterile SAL) was injected i. p. and bilateral intracranial injections of ‘aged'AB 12-28 (2.5 microl/hemisphere) were made into the IMM. 24 hours after training using 100% MeA, chicks were tested for memory retention by presentation of a dry chrome bead and birds scored as avoid (indicating memory retention) or peck (amnestic). The percentage avoidance of chicks untreated with AB or CLN was 82% and those injected i. c. with AB and i. p. SAL, memory retention fell to 52%. However, in birds injected with i. p. CLN and i. c. AB the percentage avoidance for the bead rose to 84% (Chi=55.63; p = 0.001) demonstrating that colostrinin can alleviate AB-induced memory impairment. Funding from ReGen.
Theme	Neurological and psychiatric conditions Neurodegenerative disorders / Alzheimers disease: Other