Poster board 442 - Mon 10/07, 16:00 - Hall Y
Session 130 - Alzheimer's disease II
Abstract A130.2, published in FENS Forum Abstracts, vol. 3, 2006.
Ref.: FENS Abstr., vol.3, A130.2, 2006
|Author(s)||Banks D. & Stewart M. G.|
|Biol. Sci., Open Univ., Milton Keynes, UK|
|Title||Colostrinin (CLN) alleviates beta amyloid-induced (AB) memory impairment in day-old chicks (Gallus domesticus).|
|Text||Colostrinin is a biologically active proline-rich polypeptide complex being developed by ReGen Therapeutics Plc (London, UK) that has therapeutic potential for a number of human diseases particularly Alzheimers. When injected into the mesopallium intermediomediale (IMM), an area that plays a key role in the initial stages of memory formation in the chick, CLN has a strong effect on memory enhancement when compared with control i. c. saline (SAL) injections (Stewart MG, & Banks D, 2006, Neurobiol. Learn. Memory, in press). The present experiments were designed to examine the efficacy of CLN to alleviate beta amyloid-(AB) -induced memory impairment using a single one-trial learning paradigm, avoidance of a bitter tasting substance (methylanthranilate, MeA). Day-old chicks were placed in pairs in aluminium pens kept at 25-28oC, illuminated with a 25W red bulb and after 1h of equilibration were pre-trained by two 10s presentations of a dry white bead. Only chicks that pecked the bead twice were included in subsequent experiments. Chicks were then trained with a chrome bead dipped in 100% MeA and those that pecked the bead displayed a disgust reaction with long-term memory indicated by their subsequent avoidance of pecking at a similar but dry bead 24h later. Colostrinin (100 microg in 0.1 ml 0.9% sterile SAL) was injected i. p. and bilateral intracranial injections of aged'AB 12-28 (2.5 microl/hemisphere) were made into the IMM. 24 hours after training using 100% MeA, chicks were tested for memory retention by presentation of a dry chrome bead and birds scored as avoid (indicating memory retention) or peck (amnestic). The percentage avoidance of chicks untreated with AB or CLN was 82% and those injected i. c. with AB and i. p. SAL, memory retention fell to 52%. However, in birds injected with i. p. CLN and i. c. AB the percentage avoidance for the bead rose to 84% (Chi=55.63; p = 0.001) demonstrating that colostrinin can alleviate AB-induced memory impairment. Funding from ReGen.
|Theme||Neurological and psychiatric conditions
Neurodegenerative disorders / Alzheimers disease: Other